INTERFERON-GAMMA RELEASE ASSAYS FOR DIAGNOSIS OF INVASIVE FUNGAL INFECTIONS

摘要

Methods, kits, and diagnostic devices are disclosed for diagnosing an invasive fungal infection in a subject by measuring a T-cell interferon gamma (IFN-γ) response after exposure to a fungal antigen.

主权项

1. A method for diagnosing an invasive fungal infection in a subject, the method comprising:
(a) obtaining a biological sample comprising at least one peripheral blood mononuclear cell (PBMC) from a subject; (b) adding at least one antigen from an invasive fungus suspected to be infecting the subject to the biological sample; (c) detecting a T-cell interferon gamma (IFN-γ) response by detecting the IFN-γ produced by the biological sample; (d) comparing the levels of the IFN-γ produced by the biological sample to levels of IFN-γ produced in a control sample that contains no antigen; and wherein a significant difference between the levels of the IFN-γ produced by the biological sample to the levels of IFN-γ produced in the control sample indicates that the subject is infected with the invasive fungus.