NUCLEIC ACIDS AND METHODS FOR DETECTING PATHOGENS AND BENEFICIAL MICROORGANISMS

摘要

The present invention relates to nucleic acids and methods for detecting pathogens and beneficial microorganisms.

主权项

1. A method for detecting by hybridization techniques at least one pathogen and/or one beneficial microorganism in a sample, said method comprising the use of a set of nucleic acids comprising or consisting of:
(i) the sequences selected from the group consisting of:GGGTTGCTTGCTTGCGAGCTCC;(SEQ ID No 1)CTGGTGTTTGGACTCGCCTTAAAAC;(SEQ ID No 2)GCACATATTTTGCGCTTTGTATCAGG;(SEQ ID No 3)TCCGCCAGGGAARACCAAAACTCT;(SEQ ID No 4)CGGAGCGCGGGCCGTCGCG;(SEQ ID No 5)ACTTATACCCAAAACGTTGCCTCG;(SEQ ID No 6)CATTATCGAGTTTACGCTCCATAAC;(SEQ ID No 7)GCGCAGCTATTAGATCTACGGTG;(SEQ ID No 8)GCAAGGCTGGAGTATTTTATTACCCT;(SEQ ID No 9)CCGTGGCCTTGTTGCCACGCCC;(SEQ ID No 10)ATTGGGGCCTTGTTGCCACACCC;(SEQ ID No 11)TTTTGCGCTTTGTCCAGTTGCGG;(SEQ ID No 12)GATTCGTCGCCCCCCCTCCTGG;(SEQ ID No 13)GACCTTATTCAAACCTTTTTTTCAGTT;(SEQ ID No 14)GCTGTTGGGGACCGGCTCACCCG;(SEQ ID No 15)TGACCGTTGTCACGAGACGACTTTAT;(SEQ ID No 16)CTTGGTGTTGGGAGCTGCAGTCC;(SEQ ID No 17)CCATTGCGTAGTAGTAAAACCC;(SEQ ID No 18)AACGCGCTTCGTTCGGAGGCTT;(SEQ ID No 19)TTCAACCCTCAAGCCCCCGGGTTTG;(SEQ ID No 20)CGAAGTAGTGATATTCCGCATCGG;(SEQ ID No 21)GTTAGGGGGTCCCCTCTCCGG;(SEQ ID No 22)CTCGGTCTCGAGCCGCCGG;(SEQ ID No 23)ACACCCCATTGAACCTATTTATTTTYAA;(SEQ ID No 24)ACATCTCGCGCTTTGCATTCAGAA;(SEQ ID No 25)CGATTTTGGGGGGTGGCTAGTGC;(SEQ ID No 26)CTCTGAGTACGAAAAGAACCTGAAA;(SEQ ID No 27)TGCTGGCTCTTCTAGAGTCGGCTC;(SEQ ID No 28)GGCCAGACGACAGCCATAAACC;(SEQ ID No 29)CGCCGGTGGACTACCTAAACTCT;(SEQ ID No 30)CGTCGTCGCTGTTCGCAAGGAC;(SEQ ID No 31)TCGGGCAACGGAACCAGGCGC;(SEQ ID No 32)GTTGCCTCGGCGGGCACGGC;(SEQ ID No 33)CCAGTTATATAGGCACCCAATAAGCC;(SEQ ID No 34)CACCAAACCAGCTTGGGAAACCTT;(SEQ ID No 35)GTCACGTGGTCTTGGTTTTGAA;(SEQ ID No 36)CGCAACCGGGAGCCGCGGCGCGG;(SEQ ID No 37)TGGACTGGCTTCGGCTAGACTGG;(SEQ ID No 38)ACCATAGCTCAGTTGCTTGGCTTTT;(SEQ ID No 39)TGGCGAATGTTTGGACTTCGGTCT;(SEQ ID No 40)GCGCAAGCTGGGGTGGRCGAG;(SEQ ID No 41)GAACTTGTGTCTCTGCGGCGCG;(SEQ ID No 42)AACTGGTGAACCGTAGCTGTGTGGT;(SEQ ID No 43)CCGTACATTAAACTTGACTTTCTTCC;(SEQ ID No 44)GGAACTCCACCCTTGAATACACTG;(SEQ ID No 45)CGACCCCTTTTATAATTCACCCAAC;(SEQ ID No 46)CTAAAAAACCCCTCATAACCTTTTTTT;(SEQ ID No 47)CTCCTAAAACCCAATATCTTATTTTTAAG;(SEQ ID No 48)CAATACTGCCATCTTGTTTTTGAAGG;(SEQ ID No 49)ATACTTGCCATCTTTTTGGAAGG;(SEQ ID No 50)CCTAAAAACCCCCCTTATCA;(SEQ ID No 51)CAACCTTTTTGGAGTATTCTAATGAT;(SEQ ID No 52)ATACTGCCATCTTATTKAAGGGAGAC;(SEQ ID No 53)CAGGGCTATCCCCCTGCCAGG;(SEQ ID No 54)GGAGGGTTGCGCACTTTGTGCGTG;(SEQ ID No 55)GACGCCCTGTTTTCGGATAGGG;(SEQ ID No 56)TGGCGTGCGTTTGCTTGCGCTTC;(SEQ ID No 57)CTATACTCCGAGAACGAAAGTTTTTGG;(SEQ ID No 58)TAGTAGTGTGTGTRGCACGTTGTC;(SEQ ID No 59)TGTTTTTGTTTTGTGGAAATACGCTGTTT;(SEQ ID No 60)GTAGAATTTTGCTGCTCTTGGGCG;(SEQ ID No 61)CTGTGTAGTCAGGGATGGAATGTGC;(SEQ ID No 62)TGAACGCATCATGTTGCTTCGGG;(SEQ ID No 63)CTGGCTTTTGTTTTGGATTTGGAGGT;(SEQ ID No 64)CAGCGACAACCGACTCTAAGTTCA;(SEQ ID No 65)CTCGTGAAACACATGAAGTCTGAG;(SEQ ID No 66)TCGGCGCCCCAGGAGAAATCCT;(SEQ ID No 67)AGTCCATGTCCGCAATGGCAGG;(SEQ ID No 68)AACACATACCTCTCGTTACAGGGTC;(SEQ ID No 69)GAGCTGCTCTTCGGGGCCTTGTAT;(SEQ ID No 70)CTTGTATGCGCGCCAGAGAATATCA;(SEQ ID No 71)TATTGGGCGTCCGCGGGGGA;(SEQ ID No 72)GCGGAGTTCACGAGCCCTCAC;(SEQ ID No 73)GCGCCTTGTCTCTCGCGAGAC;(SEQ ID No 74)AGCTGGATCTCAGTGTTATGCTTGG;(SEQ ID No 75)GTGCCTCTCGGGGCTTCTGCCG;(SEQ ID No 76)CCTGTGTAGTAATGCTTAGCTTACAC;(SEQ ID No 77)CTACGGAGGGGTGGCTGCGTTG;(SEQ ID No 78)CTACGGAGGGGTGGCTGCGTTG;(SEQ ID No 79)ACAGCTCTGAGCAAAAATTCAAAATG;(SEQ ID No 80)GTCCAATGTAGGCGCAGCGTAA;(SEQ ID No 81)ATTAGCTGGAACCTCTTGTGGACC;(SEQ ID No 82)ATTAGCTGGAACCTCTTGTGGACC;(SEQ ID No 83)CTCATTAAACAATTTTTTCTTATAAAGATTG;(SEQ ID No 84)GTCATTGCACTCAGGTAGACGTAACA;(SEQ ID No 85)CTTTTTTCTTTTGGAAAAGGTTGACG;(SEQ ID No 86)ACAGACAATTTTATTGAACACTTTTT;(SEQ ID No 87)TTTGAAGAGTTGGCGGATCGGTAT;(SEQ ID No 88)ACGGACAATTTTATTTAACACTTTTG;(SEQ ID No 89)GCCCTTAAAAKCAGTGGCGGACC;(SEQ ID No 90)TAATACATGCAAGTCGAGCGGACAGAT;(SEQ ID No 91)GGGTACTTACCTAATACGTGAGTAT;(SEQ ID No 92)GAAGGGTARTGTCTTAATACGGCATT;(SEQ ID No 93)GGAAGGGYAGTGTGTTAATAGCAC;(SEQ ID No 94)ATGACTGTCCATCGCATGGTGGAT;(SEQ ID No 95)CTGCGGATCGCATGGTCTGCG;(SEQ ID No 96)AATACTTCTCCTCGCATGGGGAGG;(SEQ ID No 97)ATGACACACGACCGCATGGTCTGT;(SEQ ID No 98)CACCGGAAACGGCCAGAGATGGTCG;(SEQ ID No 99)andGAGTGTGGTAGAGGATGGCGGAA;(SEQ ID No 100) (ii) the sequences having at least 80% identity with SEQ No 1 SEQ ID No 2, SEQ ID No 3, SEQ ID No 4, SEQ ID No 5, SEQ ID No 6, SEQ ID No 7, SEQ ID No 8, SEQ ID No 9, SEQ ID No 10, SEQ ID No 11, SEQ ID No 12, SEQ ID No 13, SEQ ID No 14, SEQ ID No 15, SEQ ID No 16, SEQ ID No 17, SEQ ID No 18, SEQ ID No 19, SEQ ID No 20, SEQ ID No 21, SEQ ID No 22, SEQ ID No 23, SEQ ID No 24, SEQ ID No 25, SEQ ID No 26, SEQ ID No 27, SEQ ID No 28, SEQ ID No 29, SEQ ID No 30, SEQ ID No 31, SEQ ID No 32, SEQ ID No 33, SEQ ID No 34, SEQ ID No 35, SEQ ID No 36, SEQ ID No 37, SEQ ID No 38, SEQ ID No 39, SEQ ID No 40, SEQ ID No 41, SEQ ID No 42, SEQ ID No 43, SEQ ID No 44, SEQ ID No 45, SEQ ID No 46, SEQ ID No 47, SEQ ID No 48, SEQ ID No 49, SEQ ID No 50, SEQ ID No 51, SEQ ID No 52, SEQ ID No 53, SEQ ID No 54, SEQ ID No 55, SEQ ID No 56, SEQ ID No 57, SEQ ID No 58, SEQ ID No 59, SEQ ID No 60, SEQ ID No 61, SEQ ID No 62, SEQ ID No 63, SEQ ID No 64, SEQ ID No 65, SEQ ID No 66, SEQ ID No 67, SEQ ID No 68, SEQ ID No 69, SEQ ID No 70, SEQ ID No 71, SEQ ID No 72, SEQ ID No 73, SEQ ID No 74, SEQ ID No 75, SEQ ID No 76, SEQ ID No 77, SEQ ID No 78, SEQ ID No 79, SEQ ID No 80, SEQ ID No 81, SEQ ID No 82, SEQ ID No 83, SEQ ID No 84, SEQ ID No 85, SEQ ID No 86, SEQ ID No 87, SEQ ID No 88, SEQ ID No 89, SEQ ID No 90, SEQ ID No 91, SEQ ID No 92, SEQ ID No 93, SEQ ID No 94, SEQ ID No 95, SEQ ID No 96, SEQ ID No 97, SEQ ID No 98, SEQ ID No 99 and SEQ ID No 100, or (iii) the complementary sequences of (i) or (ii).