| 摘要 |
A method for preparing allose by using ribose 5-phosphate isomerase is provided to improve preparation yield by inhibiting production of by-products and improve preparation specificity to allose without addition of metal ions, so that the allose prepared is useful for inhibiting growth of cancer cells and damage of internal organs caused by ischemia. A D-allose is prepared by reacting D-psicose with ribose 5-phosphate isomerase isolated from Clostridium thermocellum, which is mass produced by (1) preparing a recombinant expression vector, pQE30/ribose 5-phosphate isomerase, containing ribose 5-phosphate isomerase gene, (2) culturing a transformed microorganism, Escherichia coli BL21(DE3) pQE30/ribose 5-phosphate isomerase, (3) inducing the expression of the ribose 5-phosphate isomerase gene, and (4) separating and purifying the expressed recombinant protein.
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