<i>Lactobacillus plantarum </i>cells with improved resistance to high concentrations of ethanol

摘要

Cycloserine resistant mutants of lactic acid bacteria characterized by having improved resistance towards ethanol. The cycloserine resistant mutants of lactic acid bacteria can e.g. be used for malolactic fermentations of wine (i.e. including sparkling wine such as Cava/champagne) having high alcohol levels.

主权项

1. A method for producing a wine, comprising administering to a grape juice or wine a Lactobacillus plantarum composition comprising from 104 to 1014 CFU/g Lactobacillus plantarum cells, wherein the Lactobacillus plantarum cells:
(i) have a resistance to D-cycloserine, such that the amount of D-cycloserine required to reduce the OD600 measured growth of the Lactobacillus plantarum cells by 50% after 24 hours growth at 18° C. in Grape Juice GJ-5 medium as compared to growth in Grape Juice GJ-5 medium without added D-cycloserine is higher than 70 μg/ml D-cycloserine; and (ii) wherein the D-cycloserine resistant Lactobacillus plantarum cells have a resistance towards ethanol, such that the cells can grow to an OD600 of at least 0.8 after 3 days incubation at 25° C. in Grape Juice GJ-5 medium with 11% ethanol.