ENHANCED HETEROLOGOUS PRODUCTION OF LIPOXYGENASES

摘要

The invention is directed to the enhanced expression and purification of lipoxygenase enzymes. These enzymes are of wide-spread industrial importance, often produced in heterologous microbial systems. Preferably, the lipoxygenase produced by the methods of the invention is a plant-derived enzyme and expressed at high-levels in a microbial system that includes a protease-deficient host and one or more chaperone expression plasmids. The invention is also directed to amino acid and nucleic acid fragments of the lipoxygenase enzyme including fragments in expression constructs encoding all or a portion of one or more lipoxygenase genes. The invention is also directed to methods of manufacturing bread and other food and also non-food products with lipoxygenase manufactured by the methods of the invention.

主权项

1. A method of producing lipoxygenase enzyme comprising:
providing a nucleic acid expression construct within a host microorganism, wherein the construct encodes a plant-derived lipoxygenase enzyme; providing one or more chaperone plasmids within the host microorganism; and inducing expression of a lipoxygenase polypeptide encoded by the construct.