发明名称 |
Method of increasing efficiency of directed evolution of a gene using phagemid |
摘要 |
An improved method of mutanting DNA is provided comprising mutating a gene bearing phagemid in a host and infecting the host cell with a suitable helper phage to initiate packaging of the DNA into a phagemid. The packaged phagemid is then used to infect a second host cell. The method facilitates more efficient screening for genetic diversity. Also, the gene of interest is mutated and separable from the host cell which undergoes the mutational event reducing false positive results or other artifacts of general mutation techniques.
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申请公布号 |
US6156509(A) |
申请公布日期 |
2000.12.05 |
申请号 |
US19970968627 |
申请日期 |
1997.11.12 |
申请人 |
GENENCOR INTERNATIONAL, INC. |
发明人 |
SCHELLENBERGER, VOLKER |
分类号 |
C12N15/09;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N9/86;C12N15/01;C12N15/10;C12P21/02;(IPC1-7):C12Q1/68 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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