| 摘要 |
PROBLEM TO BE SOLVED: To provide a lumbrokinase dry powder containing a much active component, capable of controlling product quality and industrially mass-producible, and to provide a method for producing the same. SOLUTION: The lumbrokinase dry powder contains≥60 wt.% protein and has 14,000-28,000 U/mg enzyme activation value. The lumbrokinase dry powder is produced by washing live earthworm with water, putting the washed earthworm in a sugar or sodium chloride, leaving to stand at room temperature, stimulating the earthworm to spew the secretion in the digestive tract, adding water to the earthworm body to cause cytoclasis, preparing a homogenate slurry thereof, removing crude residue from the obtained homogenate slurry, performing supernatation, collecting eluant by using an anion exchange chromatography column or an affinity chromatography column, concentrating, desalting and, degerming, and treating the degermed supernatant by lyophilization to obtain the powder. COPYRIGHT: (C)2005,JPO&NCIPI
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