METHOD FOR DETECTING GENES SENSITIVE TO LOW-LEVEL IONIZING RADIATION, AND GENE DETECTED BY THE METHOD

摘要

A method for detecting genes sensitive to low-level ionizing radiation and genes detected by the method. More specifically, genes sensitive to low-level ionizing radiation and related to suppressing thymic cancer, discovered in a carcinogenic entity and verified in a normal entity are detected by subjecting a cancerous AKR/J mouse and a normal ICR mouse to low-level radiation. Thymus is collected therefrom, immunogenic and apoptotic genes are classified via microarray processing of the thymus. The genes are amplified and the levels of gene expression are measured. Thus, a gene having a specific reaction to radiation to be accurately detected by preventing the interference of confounding variables.

主权项

1. A method for detecting a gene sensitive to a low level of radiation, identified in a cancer-induced individual and verified using a normal individual, the method comprising the steps of:
irradiating a cancer-induced AKR/J mouse and a normal ICR mouse with a low level of radiation; extracting thymi from the AKR/J mouse and the ICR mouse; analyzing the thymi by a microarray analysis; selecting an immunity-related or apoptosis-related gene from the microarray analysis through a Venn diagram analysis; and amplifying the gene and measuring an expression level of the gene.