METHOD AND SYSTEM FOR SEQUENCING IN CHARACTERIZATION OF ANTIBODY BINDING BEHAVIOR

摘要

A method and system for characterization of antibody-bound targets by sequencing of synthetic oligonucleotides bound to the antibodies, the method including: receiving a sample having a set of antibodies; conjugating each of the set of antibodies with an oligonucleotide, thereby generating a set of oligonucleotide-conjugated antibodies; binding a first subset of the set of oligonucleotide-conjugated antibodies to a set of targets at a capture substrate; determining a sequence for at least one of: 1) each oligonucleotide of the first subset of oligonucleotide-conjugated antibodies that bind to the set of targets and 2) each oligonucleotide of a second subset of the set of oligonucleotide-conjugated antibodies that fail to bind to the set of targets; and generating an analysis of the sample from the sequences determined from at least one of the first and the second subsets of oligonucleotide-conjugated antibodies.