发明名称 |
QUALITY ASSESSMENT OF CIRCULATING CELL-FREE DNA USING MULTIPLEXED DROPLET DIGITAL PCR |
摘要 |
The present invention provides a method of determining integrity and/or quantity of cell free DNA (cfDNA) in a biological sample comprising amplifying target sequences with at least a first primer/probe set and at least a second primer probe/set, amplifying the target sequences of differing lengths, and monitoring for detection of the labels of the oligonucleotide probes, and determining the integrity and/or quantity of the cfDNA based on the level of detection of the label of the oligonucleotide probe from the first primer/probe set compared to the level detection of the label of the oligonucleotide probe from the second primer/probe set. The present invention also provides methods for generating a library with the cfDNA for sequencing and analysis. |
申请公布号 |
WO2016168844(A1) |
申请公布日期 |
2016.10.20 |
申请号 |
WO2016US28159 |
申请日期 |
2016.04.18 |
申请人 |
THE TRANSLATIONAL GENOMICS RESEARCH INSTITUTE |
发明人 |
MURTAZA, Muhammed;CONTENTE-CUOMO, Tania |
分类号 |
C12Q1/68;G06F19/00;G06F19/22 |
主分类号 |
C12Q1/68 |
代理机构 |
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