| 发明名称 |
TISSUE EXTRACELLULAR MATRIX PARTICLES AND APPLICATIONS |
| 摘要 |
An apparatus in the form of a chip is provided wherein the apparatus is prepared with decellularized extracellular matrix from various tissues and can be used to investigate the cellular interactions between the ECM and the various cell types. Three dimensional culture methods for investigating decellularized extracellular matrix from various tissues and interactions with various mammalian cell types are also provided. Methods of use of cells grown using the apparatus and methods disclosed are also provided. |
| 申请公布号 |
US2016237390(A1) |
申请公布日期 |
2016.08.18 |
| 申请号 |
US201415024139 |
申请日期 |
2014.09.24 |
| 申请人 |
THE JOHNS HOPKINS UNIVERSITY |
发明人 |
Beachley Vincent;Gibson Matt;Elisseeff Jennifer |
| 分类号 |
C12M1/12;A61K35/545 |
主分类号 |
C12M1/12 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. An apparatus for culturing cells comprising:
a first functionalized substrate having at least one functionalized surface; a gel pad disposed on the functionalized surface of the first functionalized substrate; an array comprising a plurality of discrete layers of collagen positioned on top of the gel pad, each having a defined area and being substantially aligned with one another and defining a space between one another; and a layer of decellularized extracellular matrix particles (DECM) positioned on top of at least one or more of the discrete layers of collagen, wherein the DECM is capable of supporting cellular growth. |
| 地址 |
Baltimore MD US |
