Compositions, Devices, Systems, and Methods for Using a Nanopore

摘要

Devices and methods that can detect and control an individual polymer in a mixture is acted upon by another compound, for example, an enzyme, in a nanopore are provided. The devices and methods also determine (˜>50 Hz) the nucleotide base sequence of a polynucleotide under feedback control or using signals generated by the interactions between the polynucleotide and the nanopore. The invention is of particular use in the fields of molecular biology, structural biology, cell biology, molecular switches, molecular circuits, and molecular computational devices, and the manufacture thereof.

主权项

1. A method for controlling insertion of a pore molecule into a thin film of a nanopore device, the thin film separating two pools of a liquid medium, the method comprising:
(a) providing, in one of the two pools, an amount of pore molecules sufficient to form at least a single nanopore in said thin film by insertion of a pore molecule into the thin film; (b) providing a circuit comprising electrodes in the two pools, thereby providing a potential difference across the thin films, said circuit further connected to voltage control logic; and (c) detecting insertion of a pore molecule into the thin film by sensing a change in a transport property with the voltage control logic; and (d) adjusting the potential difference in response to the change in a measured transport property resulting from insertion of the pore molecule, to thereby decrease the possibility of insertion of a second nanopore in said film.