| 发明名称 |
PCR AMPLIFICATION METHODS AND KITS FOR DETECTING AND QUANTIFYING SULFATE-REDUCING BACTERIA |
| 摘要 |
A kit for optional use with a PCR method of amplification may include at least one reaction well, and an internal amplification control for a PCR amplification of an APS reductase gene having a sequence complementary to at least one sequence essentially identical to SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, and mixtures thereof. The kit may be used with a PCR method of amplifying at least one sulfur-reducing bacteria extracted from an oilfield fluid. |
| 申请公布号 |
US2016289739(A1) |
申请公布日期 |
2016.10.06 |
| 申请号 |
US201615066558 |
申请日期 |
2016.03.10 |
| 申请人 |
BAKER HUGHES INCORPORATED |
发明人 |
Lee Crystal;Sharma Neil;Reeves Angela |
| 分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A kit comprising:
at least one reaction well; an internal amplification control for a PCR amplification of an APS reductase gene having a sequence complementary to at least one sequence essentially identical to SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, and mixtures thereof; and wherein the kit is used with a PCR method of amplifying at least one sulfur-reducing bacteria extracted from an oilfield fluid. |
| 地址 |
Houston TX US |
