METHODS AND KITS FOR CAPTURING SPERM NUCLEIC ACIDS

摘要

The invention generally relates to methods and kits for capturing sperm nucleic acids from or in a biological sample. In one embodiment the method the method comprises, contacting the sample with a lysis solution, having a protamine-DNA complex, to lyse the cell and applying a protamine-specific antibody. This results in the protamine-specific antibody binding to the protamine-DNA to form a complex which may be captured, purified, or detected. Also provided are kits for carrying out the disclosed methods.

主权项

1. A method of capturing a sperm deoxyribonucleic acid (DNA) from a biological sample, comprising:
contacting a lysis solution with the biological sample, where the biological sample comprises at least a sperm cell or a sperm cell lysate, and the lysis solution comprises a protamine-DNA complex resulting in a lysed sperm cell; applying at least a protamine-specific antibody to the lysed sperm cell, wherein the protamine-specific antibody binds to the protamine-DNA complex of the lysed sperm cell to form an antibody-protamine-DNA complex; capturing the antibody-protamine-DNA complex; and optionally, detecting the sperm DNA from the captured antibody-protamine-DNA complex.