| 摘要 |
The present invention provides the means and the methods for obtaining plant materials producing large amounts of functionally active cysteine synthase (EC 2.5.1.47) that rely on an environment-friendly and health-safe enzyme production process for synthesis of L-cysteine and L-cystine, therefore suitably accounting among the c-GMP standards for production of the aforementioned compounds, namely of non-human and non-animal origin. Described is a recombinant DNA molecule comprising a nucleic acid molecule encoding a protein having cysteine synthase activity, wherein the said nucleic acid molecule is operably linked to regulatory elements allowing the expression of the nucleic acid molecule in plant cells. The present invention also provides a vector harboring the plant expression cassette comprising the said recombinant DNA molecule, as well as plant cells, plant tissues and plants transformed therewith. The present invention further describes the use of the aforementioned recombinant DNA molecule and vector in plant cell culture, plant tissue culture, and plant breeding; further presented are examples of the use of the said plant cells, plant tissues, plants, as well as the extracts from these plant raw materials for biosynthetic production of L-cysteine and/or L-cystine compounds being valuables in a broad range of applications, such as for production of additives or ingredients used in agroindustrial, cosmetic and pharmaceutic processes. |
