Methods for preventing a chytrid infection in a culture of <i>Haematococcus pluvialis </i>using hydrogen peroxide

摘要

Methods of treating contamination, particularly fungal contamination, in cultures of Haematococcus pluvialis with hydrogen peroxide are described herein. The method comprises detecting the contamination and then dosing the culture with a concentration of hydrogen peroxide based on the stage of the cells in the culturing process and at a frequency to increase the likelihood of the cells surviving until the process of accumulating carotenoids, such as astaxanthin, is complete.

主权项

1. A method of preventing a chytrid infection in a culture of Haematococcus pluvialis, comprising:
a. Culturing a population of Haematococcus pluvialis cells in a liquid culture medium; b. Determining a number of Haematococcus pluvialis cells infected with chytrids in the culture; c. Contacting the culture with hydrogen peroxide when the percentage of Haematococcus pluvialis cells infected with chytrids is less than 10% of the total cells; d. Continuing to culture the Haematococcus pluvialis cells; and e. Verifying that a percentage of Haematococcus pluvialis cells infected with chytrids is maintained at a level less than 10% of the total cells after contact with the hydrogen peroxide.