| 摘要 |
Fabrication of arrays, including glycan arrays, that combines the higher sensitivity of a layered Si-SiO2 substrate with novel immobilization chemistry via a "click" reaction. The novel immobilization approach allows the oriented attachment of glycans on a "clickable" polymeric coating. The surface equilibrium dissociation constant (KD) of Concanavalin A with eight synthetic glycans was determined using fluorescence microarray. The sensitivity provided by the novel microarray substrate enables the evaluation of the influence of the glycan surface density on surface KD values. The interaction of carbohydrates with a variety of biological targets, including antibodies, proteins, viruses and cells are of utmost importance in many aspects of biology. Glycan microarrays are increasingly used to determine the binding specificity of glycan-binding proteins. The click polymers can be prepared in different forms such as soluble polymers, hydrogels, and multi-layers. The polymers can be prepared directly by copolymerization or by copolymerization to form a pre-polymer which is then reacted to form the target polymer. Other uses include separations, including electrophoretic separations. |
