Regenerative materials

摘要

Methods of making tissue fillers are provided. In certain embodiments, the tissue is flake-like and has regenerative properties.

主权项

1. A method for preparing a tissue matrix composition comprising:
selecting a collagen-based tissue matrix; contacting the tissue matrix with a cryoprotectant solution; freezing the tissue matrix and cryoprotective solution; cutting the frozen tissue matrix and cryoprotective solution into a plurality of cut tissue pieces having irregular size and shape, wherein the temperature of the tissue matrix ranges from −10 ° C. to −40° C. for the cutting step and wherein the cut tissue has a size distribution ranging from 0.2-5 mm in length, 0.2-3 mm in width, and 0.02-0 3 mm in thickness; placing the plurality of cut tissue pieces into a liquid to form a suspension of interconnected irregularly shaped particles, and removing liquid from the suspension to form a device having inter-connected channels that permit body fluid to flow freely therethrough.