Generation of Endocrine Progenitor Cells from Human Pluripotent Stem Cells Using Small Molecules

摘要

The present invention relates to differentiation of stem cells into a homogeneous endocrine progenitor cell population suitable for further differentiation into pancreatic beta-cells. The present invention provides methods for obtaining NGN3/NKX2.2 double positive endocrine progenitor cells by exposing precursor cells to a TGF-β type I receptor inhibitor, a BMP antagonist, an adenylate cyclase activator and nicotinamide and/or exposing to the precursor cells to a selection of small molecules.

主权项

1. A method for obtaining endocrine progenitor cells, comprising exposing a cell population comprising pancreatic endoderm cells to a basal medium comprising
a TGF-β type I receptor inhibitor, noggin, an adenylate cyclase activator, and nicotinamide.