Systems, devices and methods for multiplexed diagnostics

摘要

Exemplary embodiments provide diagnostic devices, systems and methods for determining the presence or absence of one or more markers or characteristics in one or more samples. An exemplary diagnostic device may display a first two-dimensional machine-readable output to indicate the presence or absence of a first characteristic in a sample. Similarly, the exemplary diagnostic device may display a second two-dimensional machine-readable output to indicate the presence or absence of a second characteristic in a sample. An image capture device may be used to automatically detect the two-dimensional machine-readable output appearing in the diagnostic device. A computational device may be used to automatically determine whether the presence or absence of the first characteristic and/or the second characteristic based on the two-dimensional machine-readable output displayed in the diagnostic device.

主权项

1. A diagnostic device for detecting a presence or absence of one or more substances in one or more samples, the diagnostic device comprising:
a microfluidic network layer, including:
a first set of input nodes for introduction of the one or more samples to the diagnostic device;an output region, including
a first set of output nodes arranged to detect the presence or absence of a first substance in the one or more samples,a second set of output nodes arranged to detect the presence or absence of a second substance in the one or more samples, anda two-dimensional machine-readable pattern with one or more apertures and one or more solid portions overlaid over the first set of output nodes and the second set of output nodes to provide a machine-readable output indicating the presence or absence of the first substance and/or the second substance in the one or more samples; anda plurality of microfluidic channels extending between the first set of input nodes and the first and second sets of output nodes.