Modified biotin-binding protein, fusion proteins thereof and applications

摘要

The disclosure provides modified biotin-binding proteins which can be expressed in soluble form in high yield in bacteria. Also provided are fusion proteins comprising the modified biotin-binding protein and an antigen. The disclosure further provides non-hemolytic variants of alpha-hemolysin from S. aureus and fusion protein comprising non-hemolytic variant of alpha-hemolysin and a biotin-binding domains. Immunogenic compositions comprising the proteins are also disclosed and use of such immunogenic compositions for inducing an immune response or for vaccinating a subject are also disclosed.

主权项

1. A vaccine composition comprising a soluble biotin-binding protein consisting essentially of an amino acid sequence having at least 85% sequence identity to SEQ ID NO: 1, wherein the biotin-binding protein has a higher solubility than the biotin-binding protein of SEQ ID NO: 4.