| 发明名称 |
Method for purifying interleukin 1 receptor antagonist (IL-1ra) proteins |
| 摘要 |
A method is described for releasing a soluble or membrane associated intracellular protein of interest (POI) comprising the steps of: providing a cell comprising a soluble or membrane associated intracellular POI; contacting the cell with a membrane extracting composition; and causing the POI to be released from the cell under conditions sufficient for the specific release of the POI and in a soluble form.
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| 申请公布号 |
EP1944373(A2) |
申请公布日期 |
2008.07.16 |
| 申请号 |
EP20080004919 |
申请日期 |
2000.11.24 |
| 申请人 |
DANISCO A/S |
发明人 |
JOHANSEN, CLAUS LINDVALD;KJAERULFF, SOREN;MADRID, SUSAN MAMPUSTI;PEDERSEN, HENRIK;POULSEN, CHARLOTTE HORSMANS;ZARGAHI, MASOUD RAJABI |
| 分类号 |
C12N15/09;C12N15/53;C07K14/00;C07K14/39;C12N1/06;C12N9/04;C12N15/81;C12P21/00;C12P21/02;C12Q1/02;C12Q1/68;C12R1/78 |
主分类号 |
C12N15/09 |
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