Compositions and systems for forming crosslinked biomaterials and associated methods of preparation and use

摘要

Disclosed is a method of forming a gel comprising the steps of: (a) providing a dry powder composition comprising: a first component having a polyalkylene core substituted with m nucleophilic groups, where m is at least 2; and a second component having a polyalkylene core substituted with n electrophilic groups, where n is at least 2 and m+n>4; wherein the nucleophilic and electrophilic groups are nonreactive in a dry environment, and wherein, the nucleophilic group is a sulfhydryl, and the electrophilic group is selected from maleimido, substituted maleimido, haloalkyl, epoxy, imino, aziridino, ester derivatives of p-nitrophenol, p-nitrothiophenol and pentafluorophenol; N-hydroxyphthalimide esters, N-hydroxysuccinimide esters, N-hydroxysulfosuccinimide esters, and N-hydroxyglutarimide esters; esters of 1- hydroxybenzotriazole; 3-hydroxy-3,4-dihydro-benzotriazin-4-one; 3-hydroxy-3,4-dihydro-quinazoline-4-one; carbonylimidazole derivatives; acid chlorides; ketenes; and isocyanates; and (b) dissolving the composition in a first solution having a pH within the range of about 2.1-2.3 to form a homogeneous solution, and (c) adding a second solution having a pH within the range of about 6.0 to 11.0 to the homogeneous solution to form a gel. Also disclosed is a kit containing the powder composition described above, a solution with pH of 2.1-2.3, and a solution with a pH of 6.0-11.0, each packaged separately and suitable for combining immediately prior to use.