发明名称 |
METHOD FOR SCREENING CATALYTIC PEPTIDES USING PHAGE DISPLAY TECHNOLOGY |
摘要 |
A method for screening catalytic peptides using phage display technology is disclosed. A compound is exposed to a phage library. If a peptide in the library catalyzes a reaction, a gel is formed about the phage that displays the peptide. The gel, including the first phage, is separated from un-reacted phages and released from the gel. The phage is then replicated and analyzed to determine the composition of the peptide that functioned as a catalyst. |
申请公布号 |
US2016177292(A1) |
申请公布日期 |
2016.06.23 |
申请号 |
US201314902622 |
申请日期 |
2013.07.03 |
申请人 |
RESEARCH FOUNDATION OF THE CITY UNIVERSITY OF NEW YORK |
发明人 |
Matsui Hiroshi;Maeda Yoshiaki;Ulijn Rein |
分类号 |
C12N15/10 |
主分类号 |
C12N15/10 |
代理机构 |
|
代理人 |
|
主权项 |
1. A method for screening catalytic peptides using phage display technology, the method comprising:
dissolving at least a first compound in a solvent that contains a phage library that displays a plurality of peptides, the phage library including a first phage with a first peptide; forming a gel about at least the first phage as a result of a reaction of the first compound, wherein the reaction is catalyzed by the first peptide of the first phage; separating the gel, including the first phage, from un-reacted phages of the phage library; releasing the first phage from the gel; replicating the first phage by exposing the first phage to a bacterium and permitting the formation of replicated first phages; and analyzing the replicated first phages to determine a composition of the first peptide, thereby identifying the composition as a catalytic peptide that catalyzes the reaction of the first compound. |
地址 |
New York NY US |