发明名称 |
HETEROLOGOUS EXPRESSION OF TERMITE CELLULASES IN YEAST |
摘要 |
The present invention provides for heterologous expression of termite and termite-associated symbiont cellulases. The cellulases can, for example, be codon-optimized and expressed in yeast host cells, such as the yeast Saccharomyces cerevisiae. The cellulases can also be co-expressed in host cells with other cellulases. The expression in such host cells of the termite and termite-associated symbiont cellulases, and variants and combinations thereof, result in yeast with improved cellulosic activity. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems. |
申请公布号 |
US2016177283(A1) |
申请公布日期 |
2016.06.23 |
申请号 |
US201615064468 |
申请日期 |
2016.03.08 |
申请人 |
LALLEMAND HUNGARY LIQUIDITY MANAGEMENT LLC |
发明人 |
BREVNOVA Elena E.;RAJGARHIA Vineet;MELLON Mark;WARNER Anne;MCBRIDE John;GANDHI Chhayal;WISWALL Erin |
分类号 |
C12N9/42;C12N15/81 |
主分类号 |
C12N9/42 |
代理机构 |
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代理人 |
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主权项 |
1. An isolated polynucleotide comprising a nucleic acid which encodes the mature endoglucanase of SEQ ID NO: 33, wherein said nucleic acid is codon-optimized for expression in a yeast strain wherein at least one nucleotide within a sequence of 4, 5, 6, 7, 8, 9, or 10 consecutive A, T, C or G nucleotides is replaced with a different nucleotide, wherein the nucleotide replacement does not alter the amino acid sequence encoded by the polynucleotide and wherein the nucleotide replacement creates a codon that is the second most frequently used codon to encode an amino acid in the yeast strain. |
地址 |
Budapest HU |