Fluid exchange methods and devices

摘要

Methods and devices for exchanging therapeutic agents, such as cells, from one liquid medium to another liquid medium are provided. Aspects of embodiments of the methods include transferring a therapeutic agent from a first medium, such as a freezing, storage or shipping buffer, into a second medium, such as a sterile physiologically compatible buffer. In certain aspects, the transfer of the therapeutic agent from a first medium to a second medium involves the use of acoustic-focusing, or acoustophoresis. Embodiments of the subject methods may facilitate the transfer of a therapeutic agent from a storage medium to an infusion medium, and in certain embodiments include administering the therapeutic agent contained in the infusion medium to the subject. Also provided by the present disclosure are devices for practicing the subject methods.

主权项

1. A device for transferring a therapeutic agent from a first liquid to a second liquid infusion medium, the device comprising:
a) a first container comprising a first liquid comprising a therapeutic agent suspended in a first medium comprising a cryoprotectant agent; b) second container comprising a second liquid infusion medium; and c) an acoustophoresis device in fluidic communication with the first and second containers and configured to acoustophoretically move the therapeutic agent from the first liquid into the second liquid infusion medium, to produce the therapeutic agent suspended in the second liquid infusion medium; wherein the device is sterile.