FRET-based mesoporous silica nanoparticles for real-time monitoring of drug release

摘要

A compound comprising: (a) a drug carrier comprising coumarin-labeled-cysteine tethered mesoporous silica nanoparticles (MSNs) loaded with a pharmaceutically active agent, and (b) a fluorescein isothiocyanate-β-cyclodextrin (FITC-β-CD) covalently linked to said cysteine and blocking the release of said pharmaceutically active agent from said nanoparticles, wherein cleavage of said covalent linkage removes said cyclodextrin and releases said pharmaceutically active agent, and said coumarin and said fluorescin form a donor-acceptor pair so that said drug carrier has a first emission wavelength when the covalent linkage is intact and the cyclodextrin said present and a second emission wavelength after cleavage of said covalent linkage to remove said cyclodextrin and release said pharmaceutically active agent.

主权项

1. A complex comprising: (a) a drug carrier comprising coumarin-labeled-cysteine tethered mesoporous silica nanoparticles (MSNs) loaded with a pharmaceutically active agent, said cysteine tethered MSNs comprising a cleavable disulfide linkage; and (b) a fluorescein isothiocyanate-β-cyclodextrin (FITC-β-CD) positioned in proximity to said cysteine tethered MSNs to form a complex, said complex having an emission wavelength different from the emission wavelength of said cysteine tethered MSNs after cleavage of the disulfide linkage.