| 摘要 |
Disclosed are methods and apparatuses for culturing stem cell using biomaterial shell, e.g. fertilized zebrafish chorions, which may induce formation of embryonic bodies, cell proliferation, and cell differentiation to other types effectively inside the chorions without adding any inducing agents or cell differentiation stimulants. There is provided an apparatus for culturing stem cell using biomaterial shell comprising: a microwell layer having a plurality of microwells; a microhole layer having a plurality of microholes, the microhole layer being located under the microwell layer, diameter of the microholes being smaller than diameter of the microwells and diameter of the biomaterial shell; and a flow-channel layer having a plurality of flow-channels for supplying cell medium to the microholes, the flow-channel layer being located under the microhole layer, and wherein the stem cell is encapsulated by the biomaterial shell in the microwell.
|
