| 发明名称 | Methods of freezing stem cells | ||
| 摘要 | The invention is directed to a method of freezing stem cells comprising introducing the HSCs into a cell culture medium that has been conditioned with Wharton's Jelly mesenchymal stem cells (WJSCs), thereby producing a stem cell culture, and slowly freezing the stem cell culture. In other aspects, the invention is directed to compositions comprising stem cells produced by the methods provided herein. In yet other aspects, the invention is directed to pharmaceutical compositions comprising the stem cells produced by the methods provided herein. | ||
| 申请公布号 | US9402388(B2) | 申请公布日期 | 2016.08.02 |
| 申请号 | US201314069557 | 申请日期 | 2013.11.01 |
| 申请人 | National University of Singapore | 发明人 | Fong Chui Yee;Bongso Tuan Ariffeen;Lin Daniel Hao |
| 分类号 | C12N5/0797;A01N1/02;C12N5/0789 | 主分类号 | C12N5/0797 |
| 代理机构 | Hamilton, Brook, Smith & Reynolds, P.C. | 代理人 | Hamilton, Brook, Smith & Reynolds, P.C. |
| 主权项 | 1. A method of freezing CD34+ stem cells, comprising: a) introducing the stem cells into a cell culture medium that has been conditioned with Wharton's Jelly mesenchymal stem cells (WJSCs), thereby producing a stem cell culture; and b) slowly freezing the stem cell culture at a rate that maintains stem cell viability, thereby freezing the stem cells. | ||
| 地址 | Singapore SG | ||