Process for preparing L-amino acids

摘要

A process for preparing L-amino acids employing coryneform bacteria in which the AmtR regulator has been attenuated is provided. Recombinant bacteria, polynucleotides and vectors corresponding to or having the attenuated AmtR regulator are disclosed.

主权项

1. An isolated polynucleotide comprising the promoter region of the amtR gene shown in SEQ ID NO:5 and wherein the amtR gene is attenuated by at least one of
a) replacement of the guanine at position 7 of SEQ ID NO:5 by thymine, b) replacement of cytosine at position 11 of SEQ ID NO:5 by guanine, c) replacement of thymine at position 40 of SEQ ID NO:5 by guanine, d) replacement of thymine at position 45 of SEQ ID NO:5 by guanine, e) deletion of one or more of the nucleobases of position 40 to 45 of SEQ ID NO:5, f) deletion of one or more of the nucleobases between position 72 and 78 of SEQ ID NO:5, and g) replacement of one or more of the nucleobases adenine or guanine between position 72 and 78 of SEQ ID NO:5 by thymine or cytosine.