Method for assaying bilirubin and assay instrument used in bilirubin assay

摘要

A method for assaying bilirubin in which a dry reagent is used, which is capable of accelerating the reaction of bilirubin oxidase, and an assay instrument to be used in the method for assaying bilirubin, are provided. The method for assaying bilirubin is characterized in that a biological sample and a bilirubin oxidase-containing dry reagent are mixed first, and then the mixture obtained and a surfactant-containing dry reagent are mixed. An assay instrument (1) to be used in bilirubin assay is characterized in that a bilirubin oxidase and a surfactant are arranged in any of a sample supply part (11), passages (12, 13, 14), and a detection part (15) in a manner such that the bilirubin oxidase is positioned closer to the sample supply part (11) than the surfactant is.

主权项

1. A method for assaying bilirubin in a biological sample, the method comprising:
mixing the biological sample, a bilirubin oxidase-containing dry reagent, and a surfactant-containing dry reagent; and optically measuring a change caused by the mixing, wherein the mixing of the biological sample and the bilirubin oxidase-containing dry reagent is carried out before the mixing with the surfactant-containing dry reagent, and wherein the surfactant is an anionic surfactant.