| 发明名称 |
METHODS FOR MODULATING THE GLYCOSYLATION PROFILE OF RECOMBINANT PROTEINS USING SUGARS |
| 摘要 |
The present invention relates to the field of protein production, and in particular to methods and compositions for modulating glycosylation of recombinant proteins expressed in host cells. |
| 申请公布号 |
US2016185848(A1) |
申请公布日期 |
2016.06.30 |
| 申请号 |
US201514795838 |
申请日期 |
2015.07.09 |
| 申请人 |
ABBVIE INC. |
发明人 |
Hossler Patrick;McDermott Sean;Racicot Christopher;Matuck Joseph G.;Cochran Keith;Chumsae Chris M. |
| 分类号 |
C07K16/24;C12P21/00 |
主分类号 |
C07K16/24 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method of producing a composition comprising a recombinant protein with a modulated glycosylation profile, said method comprising:
culturing a host cell expressing said recombinant protein in cell culture media supplemented with a monosaccharide or an oligosaccharide, thereby producing said composition comprising said recombinant protein with a modulated glycosylation profile as compared to a control, wherein said control is a composition comprising a recombinant protein produced by culturing a host cell expressing said recombinant protein in cell culture media which is not supplemented with said monosaccharide or said oligosaccharide. |
| 地址 |
North Chicago IL US |
