ENHANCED PROTEIN EXPRESSION

摘要

The present invention relates in general to bacterial cells having a genetic alteration that results in increased expression of a protein of interest and methods of making and using such cells. Aspects of the present invention include Gram positive microorganisms, such as Bacillus species, having a genetic alteration that modifies activity of a protein encoded by the ykf operon and results in enhanced expression of a protein of interest.

主权项

1. A method for increasing expression of a protein of interest from a Gram positive bacterial cell comprising:
a) obtaining an altered Gram positive bacterial cell capable of producing a protein of interest, wherein said altered Gram positive bacterial cell comprises at least one genetic alteration that modifies activity of one or more proteins encoded by the ykf operon; and b) culturing said altered Gram positive bacterial cell under conditions such that said protein of interest is expressed by said altered Gram positive bacterial cell, wherein expression of said protein of interest is increased in said altered Gram positive bacterial cell compared to the expression of said protein of interest in a corresponding unaltered Gram positive bacterial cell grown under essentially the same culture conditions.