METHOD FOR ANALYZING MARKERS ON THE SURFACE OF VESICLES

摘要

The present invention provides a method for analyzing levels of markers of subpopulations of vesicles in a sample comprising a heterogeneous population of said vesicles, the method comprising a) contacting said sample with a set of at least two capture agents, marker of said vesicles; thereby capturing at least two subpopulations of said vesicles, b) contacting the captured at least two subpopulations of vesicles with at least two different detection agents, thereby labeling at least two markers of said at least two subpopulations of said vesicles inversely with said at least two capture agents and at least two detection agents, thereby generating an at least 2×2 matrix of signal intensities, and c) determining the levels of said at least two markers of said at least two subpopulations of said vesicles, wherein said levels of said at least two markers of subpopulations of said vesicles are determined relative to each other.

主权项

1. A method for analyzing levels of markers of subpopulations of vesicles in a sample comprising a heterogeneous population of said vesicles, the method comprising
a) contacting said sample with a set of at least two capture agents, wherein each distinguishable subset of said set of capture agents comprises a different binding agent or combination of binding agents, and wherein each different binding agent recognizes a different marker of said vesicles; thereby capturing at least two subpopulations of said vesicles of said sample; b) contacting the captured at least two subpopulations of vesicles with at least two different detection agents, wherein each different detection agent comprises a different binding agent, and wherein each different binding agent recognizes a different marker of said vesicles, and wherein at least two of said different binding agents of said at least two detection agents recognize the same at least two markers of said vesicles as two of said different binding agents of said at least two capture agents, and wherein each binding agent of said detection agents is labeled with different or the same signaling labels; thereby labeling at least two markers of said at least two subpopulations of said vesicles inversely with said at least two capture agents and at least two detection agents, thereby generating an at least 2×2 matrix of signal intensities; and c) determining the levels of said at least two markers of said at least two subpopulations of said vesicles, wherein said levels of said at least two markers of subpopulations of said vesicles are determined relative to each other.