NANO-PLASMONIC SENSOR FOR EXOSOME DETECTION

摘要

Disclosed herein are compositions and methods for exosome detection with high sensitivity by using a nano-plasmonic sensor. The nano-plasmonic sensor comprises a plurality of nanoapertures suitable for transmission measurements. The detection sensitivity is on the order of 104-fold higher than western blotting and 102-fold higher than enzyme-linked immunosorbent assay (ELISA). A portable imaging system is also disclosed, enabling rapid and high-throughput detection of exosomes. The nano-plasmonic sensor and imaging system can be useful in diagnostics.

主权项

1. A nano-plasmonic sensor for detecting exosomes comprising,
a) a transparent planar substrate; b) a metal film disposed onto one surface of the substrate, wherein the metal film comprises a plurality of nanoapertures in a predefined pattern to create a sensing area that produces surface plasmon resonance upon illumination; and c) a capture agent attached to the metal film, wherein the capture agent specifically binds to an exosome marker.