Microbubble devices, methods and systems

摘要

A hydrogel tissue engineering scaffold having microbubbles dispersed therein is disclosed. Also, a system for cell culturing including a controller and actuator to apply dynamic deformational loading to a hydrogel is disclosed. Also disclosed are methods for producing hydrogels with microbubbles and for culturing cells using hydrogels with microbubbles.

主权项

1. A cell culture apparatus comprising:
a first chemical treatment; a hydrogel scaffold having gas-filled microbubbles and cells dispersed in the hydrogel, the hydrogel scaffold being floated on top of the first chemical treatment; and a second chemical treatment applied to a top of the hydrogel scaffold.