FEEDBACK-RESISTANT HOMOSERINE TRANSSUCCINYLASES

摘要

Homoserine-transsuccinylase (MetA) that contains a mutation, relative to the wild type, and has reduced sensitivity to L-methionine and S-adenosyl-methionine (SAM) is new. Homoserine-transsuccinylase (MetA) that contains a mutation, relative to the wild type, and has reduced sensitivity to L-methionine and S-adenosyl-methionine (SAM) is new. The mutation is: (a) of Asp in sequence (1), present between positions 90 and 115; or (b) of Tyr in sequence (2), present between positions 285 and 310. Where Asp-Gly-X-X-X-Thr-Gly-Ala-Pro (1) Tyr-Gln-X-Thr-Pro (2). X : any amino acid. Independent claims are also included for: (1) the metA alleles (I) that encode the new mutants; (2) plasmids that contain (I); (3) microorganisms that include a feedback-resistant (I); and (4) method for preparing L-Met or SAM by culturing the organisms of (3). ACTIVITY : Antidepressant; Hepatotropic; Antiarthritic. No biological data is given. MECHANISM OF ACTION : None given.