PCR PRIMER LINKED TO COMPLEMENTARY NUCLEOTIDE SEQUENCE OR COMPLEMENTARY NUCLEOTIDE SEQUENCE INCLUDING MIS-MATCHED NUCLEOTIDES AND METHOD FOR AMPLIFYING NUCLEIC ACID USING SAME

摘要

The present invention relates to a primer for PCR obtained by, directly or through inosine as a linker, linking a complementary nucleotide sequence or a complementary nucleotide sequence including a mis-matched nucleotide sequence to the 5'-terminus of a forward or reverse primer; and to a PCR method comprising a step of mixing a nucleic acid template in a PCR composition containing the primer and then performing PCR on the mixture. The primer for PCR of the present invention comprises a complementary nucleotide sequence or a mis-matched nucleotide sequence in a complementary nucleotide sequence, which is linked to the 5'-terminus thereof directly or via a linker, thereby lowering the sensitivity increase due to the increase in amplification products and reducing non-specifically occurring reactions in PCR.