| 发明名称 | Protein production | ||
| 摘要 | A method for producing from host cells a heterologous polypeptide or protein comprising at least one cysteine residue in a reduced state. The method cultured the host cells in a culture medium comprising a reducing agent; and recovered the heterologous polypeptide or protein comprising at least one cysteine residue in a reduced state from the host cells or from the culture medium, where the host cells comprise a nucleic acid encoding the heterologous protein or polypeptide, and where the reducing agent is capable of permeating the plasma membrane of the host cells. | ||
| 申请公布号 | US9410177(B2) | 申请公布日期 | 2016.08.09 |
| 申请号 | US201213354748 | 申请日期 | 2012.01.20 |
| 申请人 | Thermo Fisher Scientific Baltics UAB | 发明人 | {hacek over (S)}iurkus Juozas;Neubauer Peter |
| 分类号 | C12P21/06;C12P21/02 | 主分类号 | C12P21/06 |
| 代理机构 | Thompson Hine LLP | 代理人 | Thompson Hine LLP |
| 主权项 | 1. A method for producing from host cells an active heterologous polypeptide or protein comprising at least one cysteine residue in a reduced state, the method comprising (i) culturing host cells in a culture growth medium comprising a reducing agent having a reduction potential stronger than −0.1V at pH 7 and 25° C.; and (ii) recovering the active heterologous polypeptide or protein comprising at least one cysteine residue in a reduced state from said host cells or from the culture growth medium, where the host cells comprise a nucleic acid encoding the heterologous protein or polypeptide, and where the reducing agent is capable of permeating the plasma membrane of the host cells. | ||
| 地址 | Vilnius LT | ||