MACROMOLECULAR CONJUGATES FOR VISUALIZATION AND SEPARATION OF PROTEINS AND CELLS

摘要

Macromolecular conjugates for visualization and separation of proteins and cells The present invention descripbes macromolecular water-soluble conjugates based on synthetic copolymers to which at least one affinity tag, at least one imaging probe and at least one targeting ligand are bound via covalent bonds. The macromolecular conjugate may be used in identification, visualization, quantification or isolation of proteins and/or cells both in vitro and in vivo. HPMA copolymer, i.e.poly(HPMA-co-Ma-β-Ala-TT), copolymer prepared by conventional solution radical polymerization or controlled radical copolymerization (e.g. RAFT copolymerization, reversible addition-fragmentation chain-transfer) of N-(2- hydroxypropyl)methacrylamide (HPMA) and 3-(3-methakrylamidopropanoyl)thiazolidine-2-thione (Ma-P-Ala-TT) can be preferably used as the basic copolymer.