主权项 |
1. A microfluidic chip, comprising:
a chamber having a volume and a width, the chamber being at least partially defined by a substrate, the chamber comprising an inlet having an effective cross-sectional diameter in the range of from about 1 to about 10,000 microns, and the chamber comprising an outlet, a nucleic acid concentrator disposed within the chamber, the nucleic acid concentrator being porous, the nucleic acid concentrator spans the cross-section of the chamber such that the nucleic acid concentrator is in fluid communication with both the inlet and the outlet, the inlet and the outlet being configured such that fluid exerted into the chamber enters the chamber through the inlet, contacts the nucleic acid concentrator, and exits the chamber through the exit; the nucleic acid concentrator being capable of preferentially and reversibly binding a nucleic acid and the nucleic acid concentrator comprising at least one lysing reagent; and a polymerase chain reaction reagent disposed within a first heat-sensitive encapsulant disposed within the chamber, wherein the microfluidic chip is configured to concentrate, amplify, and detect nucleic acid in the chamber, and wherein the chip is configured to permit contact between a fluid sample introduced to the chamber and the heat-sensitive encapsulant. |