DETERMINATION OF THE UNBOUND FREE FATTY ACID PROFILES IN BIOLOGICAL AND INDUSTRIAL SPECIMENS

摘要

The invention relates to determination of the profile of unbound free fatty acids (FFAu) in biological samples, such as human and animal blood specimens and plant and animal oils, by measuring the fluorescence response of sets of different fluorescently labeled fatty acid binding proteins (probes) that undergo a change in fluorescence ratio at 2 wavelengths upon binding an FFAu. Use of these profiles in human and animal disease, in basic research, in drug development and in industrial uses is disclosed.

主权项

1. A method for determining the profile of unbound FFA (FFAu) in a sample comprising the steps of:
(a) identifying a set of one or more FFAu specific probes; (b) combining each probe with a separate aliquot of the sample; (c) measuring a fluorescence index at two different emission wavelengths for each probe in the combined probe and aliquot of the sample; (d) calculating the ratio of the fluorescence index at the two wavelengths for each probe in the sample; (e) expressing each probe's ratio in terms of sum of contributions from the different FFAu to which the probes respond; and (f) solving a set of ratio equations for the profile of the FFAu concentrations.