High throughput generation and affinity maturation of humanized antibody

摘要

Compositions, methods, and kits are provided for efficiently generating and screening humanized antibody with high affinity against a specific antigen. The library of humanized antibody is generated by mutagenizing a chimeric antibody template that combines human antibody framework and antigen binding sites of a non-human antibody. Alternatively, the library of humanized antibody is generated by grafting essential antigen-recognition segment(s) such as CDRs of the non-human antibody into the corresponding position(s) of each member of a human antibody library. This library of humanized antibody is then screened for high affinity binding toward a specific antigen in vivo in organism such as yeast or in vitro using techniques such as ribosome display or mRNA display. The overall process can be efficiently performed in a high throughput and automated manner, thus mimicking the natural process of antibody affinity maturation.

主权项

1. A method for screening a library of human or humanized antibodies in yeast, comprising:
assembling a library of antibodies in yeast cells, wherein the step of assembling the library comprises: mutagenizing a VH and VL to produce a plurality of VH and VL; and introducing the plurality of VH and VL into expression vectors by homologous recombination; expressing the library of human or humanized antibodies in the yeast cells, wherein each of the human or humanized antibodies comprises a VH and a VL which form a VH/VL heterodimer in the yeast cells; contacting a specific target protein with the library of human or humanized antibodies expressed in the yeast cells; and selecting those human or humanized antibodies based on their binding to the target protein.