METHOD AND APPARATUS FOR FORMING AND GROWING EMBRYOID BODY

摘要

A method and apparatus for forming and growing an embryoid body are provided to perform formation of the embryoid body having uniformed size and shape and cell differentiation rapidly and quantitatively, and perform cell culture for a long period of time by inhibiting quality lowering of the culture medium via continuous supply of the fresh culture medium through a bottom micro-pore layer. The apparatus for forming and growing the embryoid body(EB) comprises an EB formation layer(11), a culture well layer(12) and a micro-hole membrane(13), sequentially, and optionally comprises a bottom layer under the micro-hole membrane. The method for forming and growing the embryoid body(EB) comprises the steps of: perpendicularly erecting a cylindrical vessel having diameter of 1.0-2.6 mm of which both ends are opened; filling the cylindrical vessel with the culture medium containing stem cells to hang the culture medium to the end of the cylindrical vessel by the surface tension while the culture medium maintains a hemisphere shape; and aggregating the stem cells at the end of a culture medium drop having the hemisphere shape hanged to the end of the cylindrical vessel by gravity.