CHIPS, DETECTION SYSTEMS, AND METHODS FOR MULTIPLEX PNEUMOCOCCUS SEROLOGY

摘要

According to aspects illustrated herein, there are provided a sensor chip suitable for serological detection of Streptococcus pneumoniae, a method for detecting serotypes of Streptococcus pneumoniae using the sensor chip, a detection system that includes the sensor chip, and a method for detecting serotypes of Streptococcus pneumoniae using the detection system described herein.

主权项

1. An arrayed imaging reflectometry (AIR) sensor chip suitable for serological detection of Streptococcus pneumoniae comprising:
a multilayer substrate comprising a surface comprising bound thereon antibodies or antibody binding fragments that each bind specifically to one of two or more different serotypes of Streptococcus pneumoniae, wherein the antibodies or antibody binding fragments are attached to the surface at different locations, wherein the surface forms a coating that results in destructive interference of polarized light illuminating the surface at an appropriate incident angle and wavelength in the absence of Streptococcus pneumoniae binding, and wherein exposure of the surface to a sample comprising Streptococcus pneumoniae to which the antibodies or antibody binding fragments bind produces a detectable change in reflectance at a location upon antibody or antibody binding fragment binding.