METHOD AND SYSTEM FOR SAMPLE PREPARATION

摘要

A method for preparing a sample by utilizing a shearing force in the presence of a size stabilizer to break apart the sample to obtain nucleic acid molecules in a usable size range. Once nucleic acid molecules are obtained, magnetic nanoparticles are used to concentrate and clean the nucleic acid molecules for further testing.

主权项

1. A method for preparing a nucleic acid sample using a disposable cartridge, the method comprising the steps of:
inserting a biological sample into a disrupting chamber of a cylindrical insert that is rotatably connected to a cartridge body, the disrupting chamber being fluidly connected to a first port in an edge of the cylindrical insert, the cartridge body having a syringe mold connected to a plunger; activating a disruptor that supplies sonic energy to the disrupting chamber; permitting the sonic energy to disrupt the biological sample and release nucleic acid molecules while exposing the nucleic acid molecules to a size stabilizer to produce a nucleic acid solution; permitting the nucleic acid molecules to bind to magnetic nanoparticles; rotating the cylindrical insert to align the plunger with the first port and activating the plunger to remove the nucleic acid solution from the disrupting chamber; rotating the cylindrical insert to align the plunger with a second port which is fluidly connected to a wash chamber that is proximate a magnet; activating the plunger to inject the nucleic acid solution into the wash chamber; permitting the magnet to concentrate the magnetic nanoparticles with the nucleic acid molecules at a first location of the wash chamber; activating the plunger to remove a portion of the nucleic acid solution while leaving the magnetic nanoparticles with the nucleic acid molecules at the first location; removing the nucleic acid molecules from the magnetic nanoparticles to provide prepared nucleic acid molecules.