| 摘要 |
<p>Methods of making analytical assay devices and methods of using the devices in analytical assays, such as immunoassays, are described. The analytical assay device comprises a liquid-impervious top support layer (10) that defines a rim (12) around an open port (11), and a porous reaction membrane (13) that is proximal to the top support layer (10), such that a portion of the upper surface (14) of the reaction membrane (13) and rim (12) define a sample receiving well (11). The upper surface (14) of the reaction membrane (13) is sealed to the lower surface of the top support layer (10) by a water-insoluble adhesive (19), which forms a liquid-impervious seal therebetween. An absorbent body (15) is proximal to and in liquid communication with the lower surface of the reaction membrane (13). The analytical assay device is used for the detection of a bindable target substance in a liquid sample potentially containing the target substance.</p> |
