摘要 |
The present invention provides novel recombinant pseudomonas which contain a heterologous nucleic acid molecule comprising a nucleotide sequence encoding one or more desulfurization enzymes which are components of a biodesulfurization catalyst. The invention also provides a method of desulfurizing a carbonaceous material, such as a fossil fuel, which comprises organosulfur compounds. The method comprises the steps of (1) contacting the fossil fuel with an aqueous phase containing a recombinant biocatalyst which is capable of cleaving carbon-sulfur bonds and, optionally, a flavoprotein, thereby forming a fossil fuel and aqueous phase mixture; (2) maintaining the mixture under conditions sufficient for cleavage of the carbon-sulfur bonds of the organosulfur molecules by the biocatalyst, thereby resulting in a fossil fuel having a reduced organic sulfur content; and (3) separating the fossil fuel having a reduced organic sulfur content from the resulting aqueous phase.
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申请人 |
ENERGY BIOSYSTEMS CORPORATION;DARZINS, ALDIS;XI, LEI;CHILDS, JOHN, D.;MONTICELLO, DANIEL, J.;SQUIRES, CHARLES, H. |
发明人 |
DARZINS, ALDIS;XI, LEI;CHILDS, JOHN, D.;MONTICELLO, DANIEL, J.;SQUIRES, CHARLES, H. |