NOVEL MANNOSE 6-PHOSPHATE ISOMERASE MUTANT AND USE OF THE SAME

摘要

PURPOSE: A novel mannose-6-phosphoric isomerase mutant and a method for producing L-ribose are provided to obtain L-ribose of high yield from Geobacillus thermodenitrificans. CONSTITUTION: A novel mannose-6-phosphoric isomerase mutant is prepared by converting 124th amino acid, lysine(K) and 129th amino acid, leucine(L) into arginine(R) and phenyalanine(F). A gene encoding the mutant contains a base sequence of sequence number 4. A method for preparing the mutant isomerase comprises: a step of preparing a recombinant expression vector containing the gene; a culturing a microbe transformed by the expression vector; and a step of isolating the isomerase from the microbe.