发明名称 |
Methods for generation of reporter phages and immobilization of active bacteriophages on a polymer surface |
摘要 |
Novel reporter bacteriophages are provided. Provided are compositions and methods that allow bacteriophages that are used for specific detection or killing of E. coli 0157:H7 to be propagated in nonpathogenic E. coli, thereby eliminating the safety and security risks of propagation in E. coli 0157:H7. Provided are compositions and methods for attaching active bacteriophages to the surface of a polymer in order to kill target bacteria with which the phage comes into contact. Provided are modified bacteriophages immobilized to a surface, which capture E. coli 0157:H7 and cause the captured cells to emit light or fluorescence, allowing detection of the bacteria in a sample.
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申请公布号 |
US8114622(B2) |
申请公布日期 |
2012.02.14 |
申请号 |
US20090549500 |
申请日期 |
2009.08.28 |
申请人 |
APPLEGATE BRUCE MICHAEL;PERRY LYNDA LOUISE;MORGAN MARK THOMAS;KOTHAPALLI APARNA;PURDUE RESEARCH FOUNDATION |
发明人 |
APPLEGATE BRUCE MICHAEL;PERRY LYNDA LOUISE;MORGAN MARK THOMAS;KOTHAPALLI APARNA |
分类号 |
C12Q1/70;C12N15/79;C12Q1/66 |
主分类号 |
C12Q1/70 |
代理机构 |
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地址 |
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